Biological applications of second harmonic imaging
نویسندگان
چکیده
منابع مشابه
High throughput second harmonic imaging for label-free biological applications.
Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared fe...
متن کاملConfocal Imaging of Biological Tissues Using Second Harmonic Generation
A confocal microscopy imaging system was devised to selectively detect Second harmonic signals generated by biological tissues. Several types Of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in ti...
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Molecules that have no center of symmetry are able to convert light to its second harmonic, at twice the frequency and half the wavelength. This only happens with any efficiency at very high light intensities such as are given by a pulsed laser, and because the efficiency of the process depends on the square of the intensity, it will be focal plane selective in exactly the same way as two-photo...
متن کاملSecond-harmonic imaging microscopy of living cells.
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce three-dimensional...
متن کاملSecond-harmonic imaging of plant polysaccharides.
The application of second-harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterization of biologically and commercially important plant polysaccharides. We find that imaging of cellulose requires rather high laser powers, which are above optimal values for live cell imaging. Starch, however,...
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ژورنال
عنوان ژورنال: Biophysical Reviews
سال: 2011
ISSN: 1867-2450,1867-2469
DOI: 10.1007/s12551-011-0052-9